Purification and properties of 5,6-dihydropyrimidine amidohydrolase from calf liver

Purification and characterization of dihydropyrimidine dehydrogenase from human liver.

Although dihydropyrimidine dehydrogenase has been purified to varying degrees from several species, very little is known about the human enzyme. The importance of this enzyme has recently been shown with cancer chemotherapy, particularly in patients with genetic deficiency of this enzyme. In the present study, this enzyme was purified 7800-fold to homogeneity from human liver by introducing sev...

The acylamide amidohydrolase of Candida utilis: purification and properties.

Purification and properties of thymidylate synthetase from calf thymus.

The enzyme, thymidylate synthetase, catalyzes the methylation of deosyuridylate to thymidylate, with formaldehyde as the source of the methyl group, in the presence of tetrahydrofolate. Some of the properties of this enzyme system in crude soluble protein extracts of rat thymus have previously been reported (1). Reports have also appeared of this enzyme reaction in Escherichia cozi (2, 3). The ...

extraction and acetylation of purified trypsin from bovin pancreas and study of some its physico-chemical properties

آنزیم تریپسین در شرایط قلیایی ناپایدار می باشد .و فعالیت پروتئولیتیکی تریپسین منجربه خود هضمی آن در جایگاههای خاصی می گردد. بنابر این آنزیمی با ناپایداری بالا محسوب میگردد. در سالهای اخیر موفق شدند که با ایجاد تغیرات شیمیایی با اضافه کردن فلزات خاص ، کلسیم و یا عمل استیلاسیون منجر به افزایش پایداری آنزیم تریپسین گردند. مطالعات در حال حاضر نشان می دهد که تریپسین استیله شده فعالیت آنزیمی خود را ...

Purification and properties of a 4-methylene-L-glutamine amidohydrolase from peanut leaves.

A 4-methylene-L-glutamine amidohydrolase has been partially purified from leaf extracts of 2-week germinated peanuts (Arachis hypogaea). Purification steps include DEAE-Sephacel and gel filtration chromatography as well as chromatofocusing; amidohydrolase purified 300- to 400-fold is obtained. The enzyme has an approximate molecular weight of 45,000 as determined by gel filtration chromatograph...